Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage
نویسندگان
چکیده
Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties of several indoleamines from the tryptophan metabolic pathway in the pineal gland against oxidative damage to lipids and proteins of synaptosomes isolated from the rat brain. Free radicals were generated by incubation with 0.1 mM FeCl(3), and 0.1 mM ascorbic acid. Levels of malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA), and carbonyl content in the proteins were measured as indices of oxidative damage to lipids and proteins, respectively. Pinoline was the most powerful antioxidant evaluated, with melatonin, N-acetylserotonin, 5-hydroxytryptophan, 5-methoxytryptamine, 5-methoxytryptophol, and tryptoline also acting as antioxidants.
منابع مشابه
Mitochondria: Central Organelles for Melatonin's Antioxidant and Anti-Aging Actions.
Melatonin, along with its metabolites, have long been known to significantly reduce the oxidative stress burden of aging cells or cells exposed to toxins. Oxidative damage is a result of free radicals produced in cells, especially in mitochondria. When measured, melatonin, a potent antioxidant, was found to be in higher concentrations in mitochondria than in other organelles or subcellular loca...
متن کاملMelatonin as an antioxidant: biochemical mechanisms and pathophysiological implications in humans.
This brief resume enumerates the multiple actions of melatonin as an antioxidant. This indoleamine is produced in the vertebrate pineal gland, the retina and possibly some other organs. Additionally, however, it is found in invertebrates, bacteria, unicellular organisms as well as in plants, all of which do not have a pineal gland. Melatonin's functions as an antioxidant include: a), direct fre...
متن کاملVitamin E protects against Alzheimer's amyloid peptide (25-35)-induced changes in neocortical synaptosomal membrane lipid structure and composition.
Ž . Alzheimer’s disease AD is an age-related, neurodegenerative disorder characterized by the presence of brainŽ . resident neurofibrillary tangles, senile plaques SP , and w x loss of synapses 9 . The major component of SP is the Ž . aggregated form of amyloid b-peptide A b , which is w x 39–43 amino acids in length 19 . A b is associated with free radical oxidative stress as demonstrated in m...
متن کاملElectron paramagnetic resonance investigations of free radical-induced alterations in neocortical synaptosomal membrane protein infrastructure.
Evidence is presented that free radical stress can directly induce physico-chemical alterations in rodent neocortical synaptosomal membrane proteins. Synaptosomes were prepared from gerbil cortical brain tissue and incubated with 3 mM ascorbate and various concentrations of exogenous Fe2+ for 30-240 min at 37 degrees C. Synaptosomes were then lysed and covalently labeled with the protein thiol-...
متن کاملEvaluation of antioxidant activity of natural product compounds from Platycladus orientalis leaves
Background and objectives: Natural product compounds of plants have been used since ancient times and in folklore medicine for the treatment of many diseases and illnesses. Oxygen-centered free radicals and other reactive oxygen species, which are continuously, produced in vivo, result in cell death and tissue damage. Oxidative damage caused by free radicals may be rel...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 11 شماره
صفحات -
تاریخ انتشار 2010